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KMID : 0364819920300010053
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Korean Journal of Microbiology
1992 Volume.30 No. 1 p.53 ~ p.59
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Divergence of the cbp Genes in 4-Chlorobiphenyl Catabolizing Bacteria
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Youn Deok-Joong
Han Jae-Jin
Kim Chi-Kyung
Kim Young-Soo
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Abstract
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Four bacterial strains capable of catabolizing 4-chlorobiphenyl (4CB) were isolated from the industrial waste waters. The bacterial isolates designated as P08. P20, P27, and P1242, respectively, were examined for their catabolic activities. And in order to examine molecular homology of the 4CB catabolizing genes of these bacterial isolates, Southern hybridization was conducted with bphABC genes of P. pseudoalcaligenes KF707 as a DNA probe. The metabolites of 2-hydroxy-6-oxo-6-(4´-chlorophenyl)hexa- 2,4-dienoic acid and 4-chlorobenzoate were detected to be produced by the isolates in the MM2 liquid cultures. But 4-chlorobenzoate was further catabolized to produce 4-hydroxybenzoate by DJ-12, P08, and P27, but not by P20 and P1242. As results of hybridization, homologous regions were commonly observed in Xhol fragments of 2.2 and 1.8 kb and in EcoRI fragment of 11 kb in the DJ12, P08, and P27 isolates. But in any restriction enzyme digests of the P20 and P1242 isolates, homologous region was not detected. The cbp genes of the bacteria capable of catabolizing 4CB in nature could be divided into two groups by divergences in catabolic pathway and DNA homology.
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KEYWORD
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Biodegradation of 4CB, cbp genes
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